叶酸对炔雌醚致雄性大鼠生殖损伤的保护作用研究

doi:10.11843/j.issn.0366-6964.2017.02.019

Abstract

Abstract:

The study was conducted to investigate the effects of folacin on quinestrol induced abberant reproduction in adult male rat. Twenty 8-week-old adult male SD rats were randomly divided into 4 groups after 1-week of adaptive feeding. Control group: olive oil + physiological saline; Quinestrol group: 1.0 mg•kg^-1 quinestrol; Quinestrol + folacin group: 1.0 mg•kg^-1 quinestrol + 1.1 mg•kg^-1 folacin (dissolved in saline); Folacin group: 1.1 mg•kg^-1 folacin. Quinestrol and folacin were given intragastric administration to male rats daily for 2 weeks. After the treatments, the sexual organs were weighed, semen quality were tested, antioxidant status of testes were investigated, testis tissue section were made, histopathology of testes were observed, the expressions of Caspase-3 and endothelial nitric oxide synthase (eNOS) were detected by immunohistochemical staining. The results showed that atrophy of sexual organs were induced, the weights of sexual organs were decreased, abnormal spermatogenesis were abberant, sperm quality were reduced, antioxidant enzyme activity were decreased, oxidative stress were happend in testes after the treatment of quinestrol alone. By contrast, the expressions of Caspase-3 and eNOS in the testes were increased. In the group of quinestrol and folacin treatment, oxidative stress were reduced by decreasing eNOS expression, the expression of Caspase-3 were inhibited, the sperm deformity rate and the damage of the tubule were reduced, the reproductive function of male rats were improved significantly. Compared with the control group, the testicular antioxidant enzyme activities, tissue structure and sperm quality were ameliorated to some extent in the folacin treatment group, there was no significant difference. The results clearly demonstrate for the first time that folacin effectively inhibited the damaging effects of quinestrol on reproductive function in adult male rats.

CLC Number:

S85
S814

LI Jian, CHEN Fu-ning, CHEN Yao-xing, WANG Zi-xu, DONG Yu-lan, CAO Jing,SI Li-fang,LIU Zhi-jun,ZHAO Zhan-qin. The Study of Protection of Folacin against Quinestrol Induced Reproductive Toxicity in Male Rat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 351-358.

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The Study of Protection of Folacin against Quinestrol Induced Reproductive Toxicity in Male Rat

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